Specific Detection of Subgroups I and II of Cucumber Mosaic Virus RNA with Digoxigenin-Labeled Synthetic Oligo-Deoxyribonucleotide Probes.

Satellite RNA Associated with Cucumber Mosaic Virus

Helper Virus Independent Transcription and Multimerization of A 1 Satellite RNA Associated with Cucumber Mosaic Virus 2 3 Soon Ho Choi#, Jang-Kyun Seo#, Sun-Jung Kwon# and A. L. N. Rao* 4 Department of Plant Pathology & Microbiology, University of California, Riverside, 5 California 92521-0122 6 . 7 8 9 # These authors contributed equally to this work 10 11 12 Running title: Helper virus indepe...

Simultaneous Detection of Arabis Mosaic Virus, Cherry Leafroll Virus and Cucumber Mosaic Virus with Coamplification of Plant mRNA as Internal Control for Olive Certification Programs

Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...

Hybridization and detection of digoxigenin probes on RNA blots.

This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.

Digoxigenin-labeled probes for the detection of hepatitis B virus DNA in serum.

A nonradioactive hybridization assay for the detection of hepatitis B virus (HBV) DNA in serum with a digoxigenin-labeled probe is described. The probe was sensitive, being able to detect 0.25 pg of homologous HBV DNA, equivalent to 7 x 10(4) genome copies. After extraction of DNA from clinical samples, the probe detected HBV DNA in 11 of 12 hepatitis B e antigen-positive sera and did not react...

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars